Anti beta Actin antibody [mPro7] - Loading Control (PMC201)

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Overview
Product name	Anti beta Actin antibody [mPro7] - Loading Control
Description	Mouse monoclonal [mPro7] to beta Actin
Specificity	Does not cross-react with adult cardiac, smooth, or skeletal muscle actin.
Tested applications	WB, IHC, ICC/IF, FC, IP, ELISA
Species reactivity	Reacts with: Human, Mouse, Rat, Hamster, Zebrafish. Other species are not tested, Please decide the specificity by homology.
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human beta Actin.
Positive control	This antibody gave a positive signal in the following whole cell lysates: A431; HEK293; NIH3T3.
General notes	The concentration of this antibody is batch dependent.
Properties
Form	Liquid
Storage instructions	Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer	pH: 7.40, Preservative: 0.02% Sodium azide, Constituent: PBS
Concentration	100 µg at 1 mg/ml
Purity	IgG fraction
Clonality	Monoclonal
Clone number	mPro7
Isotype	IgG2b
Applications
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. WB 1/5000 - 1/50000. IHC 1/20 - 1/200. IF 1/20 - 1/200. IP 1/5000 - 1/50000. FC Use at an assay dependent concentration.
Target
Relevance	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Alternative names	ACTB antibody, ACTB_HUMAN antibody, Actin antibody, Actin cytoplasmic 1 antibody, b actin antibody, b-actin antibody, Beta cytoskeletal actin antibody, Beta-actin antibody, etc.
Anti beta Actin antibody [mPro7] - Loading Control images
All lanes : Anti beta Actin antibody [mPro7] - Loading Control (PMC201) at 1/10000 dilution   Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate. Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate.   Lysates/proteins at 10 µg per lane. Western blot - Anti beta Actin antibody [mPro7] - Loading Control (PMC201)